Imaging Facility


Quorum Technologies Spinning Disk Confocal

  • Confocal imaging of live and fixed cells
  • Nikon Eclipse Ti - inverted microscope
  • Five laser lines: 403 nm, 445 nm, 488 nm, 561 nm, 633 nm
  • Two Hamamatsu Image EMCCD cameras
  • Photonics Mosaic System for Photoactivation and Photobleaching
  • Objectives:  60X 1.4NA, 100X 1.4NA
  • MetaMorph software
  • 20/20 temperature controlled stage


Nikon A1R SI Confocal

  • Point scanning confocal
  • Galvano and Resonant Scanners
  • Six laser lines: 405 nm, 457 nm, 488 nm, 514 nm, 561 nm, 640nm
  • Spectral Imaging
  • Simultaneous Photoactivation/Photobleaching with confocal imaging
  • Objectives:  20X .75NA, 40X 1.3NA, 60X 1.4NA, 100X 1.49NA
  • Nikon Elements software
  • 20/20 temperature controlled stage


PicoQuant TCSPC Lifetime Imaging - a module on the Nikon A1

  • Fluorescence Lifetime Imaging (FLIM)
  • Forester Resonance Energy Transfer (FRET)
  • Fluorescence Correlation Spectroscopy (FCS)


2-Color TIRF System

  • Four laser lines (405 nm, 488 nm, 561 nm, 640 nm)
  • FRAP module
  • Two Andor EMCCD cameras
  • Objectives:  60X 1.49NA, 100X 1.49NA
  • Nikon Elements software


Zeiss Axioplan II

  • Wide-field fluorescence
  • Brightfield
  • Differential Interference Contrast
  • Retiga SRV camera
  • Phylum imaging software


The resources of the Biological Sciences Imaging Facility are available to all members of the Dartmouth community and to researchers outside of Dartmouth.

All users of the equipment must be trained before using the equipment.

Please email Ann Lavanway (