Amy S. Gladfelter

Academic Appointments

Associate Professor of Biological Sciences, University of North Carolina
Adjunct Associate Professor of Biological Sciences, Dartmouth College
Member of the Cancer Mechanisms Program
Norris Cotton Cancer Center, DHMC

I am fascinated by how cells are organized in time and space. My research group studies how cells manage time to create highly variable yet accurate cell division cycles. We also look at how cells use the septin cytoskeleton to assemble functionally unique zones on the cell cortex. For our work we combine live cell microscopy and computational approaches with genetic and biochemical analyses.

Curriculum Vitae Personal Website
HB 6044
Biological Sciences
B.A. Princeton University
Ph.D. Duke University

Selected Publications

Lee C, Occhipinti P, Gladfelter AS. PolyQ-dependent RNA-protein assemblies control symmetry breaking.J Cell Biol. 2015 Mar 2;208(5):533-44. doi: 10.1083/jcb.201407105. Epub 2015 Feb 23.PMID:25713414 [PubMed - in process]

Anderson CA, Roberts S, Zhang H, Kelly CM, Kendall A, Lee C, Gerstenberger J, Koenig AB, Kabeche R, Gladfelter AS. Ploidy variation in multinucleate cells changes under stress. Mol Biol Cell. 2015 Jan 28. pii: mbc.E14-09-1375. [Epub ahead of print] PMID: 25631818 [PubMed - as supplied by publisher]

Gladfelter, AS. Cytoskeleton: cirque du septins. Curr Biol. 2014 Jun 2;24(11):R526-8. doi: 10.1016/j.cub.2014.04.030.PMID: 24892914

Bridges AA, Gladfelter, AS. Fungal pathogens are platforms for discovering novel and conserved septin properties. Curr Opin Microbiol. 2014 Aug;20:42-8. doi: 10.1016/j.mib.2014.04.004. Epub 2014 May 28. PMID:24879478 [PubMed - in process]

Bridges AA, Zhang H, Mehta SB, Occhipinti P, Tani T, Gladfelter AS. Septin assemblies form by diffusion-driven annealing on membranes. Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2146-51. doi: 10.1073/pnas.1314138111. Epub 2014 Jan 27.PMID: 24469790[PubMed - indexed for MEDLINE]

Anderson CA, Eser U, Korndorf T, Borsuk ME, Skotheim JM, Gladfelter AS. Nuclear repulsion enables division autonomy in a single cytoplasm. Curr Biol. 2013 Oct 21;23(20):1999-2010. doi: 10.1016/j.cub.2013.07.076. Epub 2013 Oct 3.PMID:24094857[PubMed - in process]

Lee C, Zhang H, Baker AE, Occhipinti P, Borsuk ME, Gladfelter AS. Protein aggregation behavior regulates cyclin transcript localization and cell-cycle control. Dev Cell. 2013 Jun 24;25(6):572-84. doi: 10.1016/j.devcel.2013.05.007. Epub 2013 Jun 13. PMID:23769973 [PubMed - indexed for MEDLINE]

Meseroll RA, Occhipinti P, Gladfelter AS. Septin phosphorylation and coiled-coil domains function in cell and septin ring morphology in the filamentous fungus ashbya gossypii. Eukaryot Cell. 2013 Feb;12(2):182-93. doi: 10.1128/EC.00251-12. Epub 2012 Nov 30. PubMed PMID: 23204191; PubMed Central PMCID: PMC3571309.

Meseroll RA, Howard L, Gladfelter AS. Septin ring size scaling and dynamics require the coiled-coil region of Shs1p. Mol Biol Cell. 2012 Sep;23(17):3391-406. doi: 10.1091/mbc.E12-03-0207. Epub 2012 Jul 5. PubMed PMID: 22767579; PubMed Central PMCID: PMC3431940.

Gerstenberger JP, Occhipinti P, Gladfelter AS. Heterogeneity in mitochondrial morphology and membrane potential is independent of the nuclear division cycle in multinucleate fungal cells. Eukaryot Cell. 2012 Mar;11(3):353-67. doi: 10.1128/EC.05257-11. Epub 2012 Jan 20. PubMed PMID: 22267774; PubMed Central PMCID: PMC3294448.

Anker JF, Gladfelter AS. Axl2 integrates polarity establishment, maintenance, and environmental stress response in the filamentous fungus Ashbya gossypii. Eukaryot Cell. 2011 Dec;10(12):1679-93. doi: 10.1128/EC.05183-11. Epub 2011 Oct 7. PubMed PMID: 21984708; PubMed Central PMCID: PMC3232731.

Spiliotis ET, Gladfelter AS. Spatial guidance of cell asymmetry: septin GTPases show the way. Traffic. 2012 Feb;13(2):195-203. doi: 10.1111/j.1600-0854.2011.01268.x. Epub 2011 Sep 19. Review. PubMed PMID: 21883761; PubMed Central PMCID: PMC3245886.

DeMay BS, Noda N, Gladfelter AS, Oldenbourg R. Rapid and quantitative imaging of excitation polarized fluorescence reveals ordered septin dynamics in live yeast. Biophys J. 2011 Aug 17;101(4):985-94. doi: 10.1016/j.bpj.2011.07.008. PubMed PMID: 21843491; PubMed Central PMCID: PMC3175061.

DeMay BS, Bai X, Howard L, Occhipinti P, Meseroll RA, Spiliotis ET, Oldenbourg R, Gladfelter AS. Septin filaments exhibit a dynamic, paired organization that is conserved from yeast to mammals. J Cell Biol. 2011 Jun 13;193(6):1065-81. doi: 10.1083/jcb.201012143. PubMed PMID: 21670216; PubMed Central PMCID: PMC3115802.

Gladfelter AS. Guides to the final frontier of the cytoskeleton: septins in filamentous fungi. Curr Opin Microbiol. 2010 Dec;13(6):720-6. doi: 10.1016/j.mib.2010.09.012. Epub 2010 Oct 9. Review. PubMed PMID: 20934902.

Nair DR, D'Ausilio CA, Occhipinti P, Borsuk ME, Gladfelter AS. A conserved G₁ regulatory circuit promotes asynchronous behavior of nuclei sharing a common cytoplasm. Cell Cycle. 2010 Sep 15;9(18):3771-9. doi: 10.4161/cc.9.18.12999. Epub 2010 Sep 13. PubMed PMID: 20930528; PubMed Central PMCID: PMC3047801.

Chen CH, DeMay BS, Gladfelter AS, Dunlap JC, Loros JJ. Physical interaction between VIVID and white collar complex regulates photoadaptation in Neurospora. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16715-20. doi: 10.1073/pnas.1011190107. Epub 2010 Aug 23. PubMed PMID: 20733070; PubMed Central PMCID: PMC2944764.

DeMay BS, Meseroll RA, Occhipinti P, Gladfelter AS. Cellular requirements for the small molecule forchlorfenuron to stabilize the septin cytoskeleton. Cytoskeleton (Hoboken). 2010 Jun;67(6):383-99. doi: 10.1002/cm.20452. PubMed PMID: 20517926.

DeMay BS, Meseroll RA, Occhipinti P, Gladfelter AS. Regulation of distinct septin rings in a single cell by Elm1p and Gin4p kinases. Mol Biol Cell. 2009 Apr;20(8):2311-26. doi: 10.1091/mbc.E08-12-1169. Epub 2009 Feb 18. PubMed PMID: 19225152; PubMed Central PMCID: PMC2669037.

Gladfelter AS, Montagna C. Seeking truth on Monte Verita. Workshop on the molecular biology and biochemistry of septins and septin function. EMBO Rep. 2007 Dec;8(12):1120-6. Epub 2007 Nov 2. PubMed PMID: 17975554; PubMed Central PMCID: PMC2267226.

Sudbery PE, Gladfelter AS. Pathocycles. Fungal Genet Biol. 2008 Jan;45(1):1-5. Epub 2007 Feb 28. PubMed PMID: 17964832.

Gladfelter AS, Sustreanu N, Hungerbuehler AK, Voegeli S, Galati V, Philippsen P. The anaphase-promoting complex/cyclosome is required for anaphase progression in multinucleated Ashbya gossypii cells. Eukaryot Cell. 2007 Feb;6(2):182-97. Epub 2006 Dec 8. PubMed PMID: 17158735; PubMed Central PMCID: PMC1797942.

Hungerbuehler AK, Philippsen P, Gladfelter AS. Limited functional redundancy and oscillation of cyclins in multinucleated Ashbya gossypii fungal cells. Eukaryot Cell. 2007 Mar;6(3):473-86. Epub 2006 Nov 22. PubMed PMID: 17122387; PubMed Central PMCID: PMC1828934.

Gladfelter AS. Nuclear anarchy: asynchronous mitosis in multinucleated fungal hyphae. Curr Opin Microbiol. 2006 Dec;9(6):547-52. Epub 2006 Oct 11. Review. PubMed PMID: 17045513.

Helfer H, Gladfelter AS. AgSwe1p regulates mitosis in response to morphogenesis and nutrients in multinucleated Ashbya gossypii cells. Mol Biol Cell. 2006 Oct;17(10):4494-512. Epub 2006 Aug 9. PubMed PMID: 16899511; PubMed Central PMCID: PMC1635347.

Gladfelter AS, Hungerbuehler AK, Philippsen P. Asynchronous nuclear division cycles in multinucleated cells. J Cell Biol. 2006 Jan 30;172(3):347-62. PubMed PMID: 16449188; PubMed Central PMCID: PMC2063645.

Gladfelter AS. Control of filamentous fungal cell shape by septins and formins. Nat Rev Microbiol. 2006 Mar;4(3):223-9. Review. Erratum in: Nat Rev Microbiol. 2006 Apr;4(4):318. PubMed PMID: 16429163.

Gladfelter AS, Kozubowski L, Zyla TR, Lew DJ. Interplay between septin organization, cell cycle and cell shape in yeast. J Cell Sci. 2005 Apr 15;118(Pt 8):1617-28. Epub 2005 Mar 22. PubMed PMID: 15784684.

Gladfelter AS, Zyla TR, Lew DJ. Genetic interactions among regulators of septin organization. Eukaryot Cell. 2004 Aug;3(4):847-54. PubMed PMID: 15302817; PubMed Central PMCID: PMC500892.

Irazoqui JE, Gladfelter AS, Lew DJ. Cdc42p, GTP hydrolysis, and the cell's sense of direction. Cell Cycle. 2004 Jul;3(7):861-4. Epub 2004 Jul 26. Review. PubMed PMID: 15190213.

Irazoqui JE, Gladfelter AS, Lew DJ. Scaffold-mediated symmetry breaking by Cdc42p. Nat Cell Biol. 2003 Dec;5(12):1062-70. Epub 2003 Nov 16. PubMed PMID: 14625559.

Gladfelter AS, Bose I, Zyla TR, Bardes ES, Lew DJ. Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p. J Cell Biol. 2002 Jan 21;156(2):315-26. Epub 2002 Jan 21. PubMed PMID: 11807094; PubMed Central PMCID: PMC2199227.

+ View 24 more

Speaking Engagements

Structure and Function of Septins, St. Goar, Germany, March 2011

Fungal Genetics Meeting, Asilomar, CA, March 2011

Gordon Research Conference, Cell Motility and the Cytoskeleton, Colby-Sawyer College, New London, NH, August 2011

Gordon Research Conference, Plant and Fungal Cytoskeleton, August 2012

AAAS meeting, Boston, MA February 2013

Selected Works and Activities

2014 Graduate Student Mentoring Award