Elizabeth F. Smith
Paul M. Dauten Jr. ProfessorAssociate Dean for the SciencesProfessor in the Molecular and Cellular Biology Graduate Program
The proper assembly and regulated function of eukaryotic cilia is critical for development and sustained human health. We use a variety of biochemical, molecular, and genetic techniques to elucidate the signal transduction pathways that regulate motor proteins responsible for ciliary beating.
Perrone, C.A., D. Tritschler, R. Bower, E. T. O'Toole, M. J. Wargo, E. F. Smith, and M. E. Porter. BOP2 encodes a novel WD-repeat protein that regulates inner arm dynein activity. (in preparation)
Vasudevan, K.K., K. Song, L.M. Alford, W.S. Sale, E.E. Dymek, E.F Smith, T. Hennessey, E. Joachimiak, P. Urbanska, D. Wloga, W. Dentler, D. Nicastro, and J. Gaertig. (2015) FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c. Molecular Biology of the Cell. Feb 15;26(4):696-710.
Wren, K.N., J.M. Craft, D. Tritschler, A. Schauer, D.K. Patel, E.F Smith, M.E. Porter, P. Kner, K.F.Lechtreck. (2013) A differential cargo-loading model of ciliary length regulation by IFT. Current Biology. Dec 16;23(24):2463-71.
DiPetrillo, C. and E.F. Smith. (2013) Methods for analysis of calcium / calmodulin signaling in cilia and flagella. Methods in Enzymology, 524: 37-57.
Dymek, E.E., T. Heuser, J. Lin, E.F. Smith, and D. Nicastro. (2012) The CSC connects three major axonemal complexes involved in dynein regulation. Molecular Biology of the Cell 23(16):3143-55
Brown J.M., C. DiPetrillo , E.F. Smith, G.B. Witman. (2012) A FAP46 mutant provides new insights into the function and assembly of the C1d complex of the ciliary central apparatus. Journal of Cell Science 125(16):3904-13
Dymek, E.E. and E.F. Smith. (2012) PF19 encodes the p60 catalytic subunit of katanin and is required for assembly of the flagellar central apparatus in Chlamydomonas. J. Cell Science 125(Pt 14):3357-66
Goduti, D., and E.F. Smith. (2012) Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub-complex members in regulating flagellar beat frequency. Cytoskeleton 69(3):179-94
DiPetrillo, C. and E.F. Smith. (2011) The Pcdp1 complex coordinates the activity of dynein isoforms to produce wild-type ciliary motility. Molecular Biology of the Cell 22(23):4527-38 .
Dymek, E.E., T. Heuser, D. Nicastro and E.F. Smith. (2011) The CSC is required for complete radial spoke assembly and wild-type ciliary motility. Molecular Biology of the Cell 22(14):2520-31
Smith, E.F. and R. Rohatgi. (2011) Cilia 2010: The surprise organelle of the decade. Science Signaling. 4:55 mr1.Straschil, U., A. Talman, D. Ferguson, K. Bunting, Z. Xu, Liz Bailes, J. Coates, T. Holder, E.F. Smith, and R. Tewari. (2010) The Armadillo repeat protein PF16 is essential for flagellar structure and function in Plasmodium male gametes. PLoS ONE . Vol. 5:10 e12901
DiPetrillo, C., and E.F. Smith. (2010) Pcdp1 is a central apparatus protein that binds Ca 2+ -calmodulin and regulates ciliary motility. Journal of Cell Biology 189(3):601-612
Works in Progress
"Regulation of Ciliary and Flagellar Motility: Understanding the Function of Calmodulin and Interacting Proteins”