Imaging Facility

Equipment

Quorum Technologies Spinning Disk Confocal

  • Confocal imaging of live and fixed cells
  • Nikon Eclipse Ti - inverted microscope
  • Three laser lines: 403, 491, 561
  • Two Hamamatsu Image EMCCD cameras
  • Photonics Mosaic System for Photoactivation and Photobleaching
  • 20/20 temperature controlled stage

Equipment

Nikon A1R SI Confocal

  • Point scanning confocal
  • Galvano and Resonant Scanners
  • Six laser lines: 405, 457, 488,514, 561, 640
  • Spectral Imaging
  • Simultaneous Photoactivation/Photobleaching with confocal imaging
  • 20/20 temperature controlled stage

Equipment

PicoQuant TCSPC Lifetime Imaging - a module on the Nikon A1

  • Fluorescence Lifetime Imaging (FLIM)
  • Forester Resonance Energy Transfer (FRET)
  • Fluorescence Correlation Spectroscopy (FCS)

Equipment

2-Color TIRF System

  • Four laser lines (405 nm, 488 nm, 561 nm, 640 nm)
  • FRAP module
  • Two Andor EMCCD cameras
  • Nikon Elements software

Equipment

Zeiss Axioplan II

  • Wide-field fluorescence
  • Brightfield
  • Differential Interference Contrast
  • Retiga SRV camera
  • Phylum imaging software

Equipment

NIkon SMZ1500 Stereo Microscope

 

  • Transmitted light
  • GFP fluorescence
  • Optronics CCD color camera
  • MetaVue imaging software

Training

The resources of the Biological Sciences Imaging Facility are available to all members of the Dartmouth community and to researchers outside of Dartmouth.

All users of the equipment must be trained before using the equipment.

Please email Ann Lavanway (ann.m.lavanwayATDartmouth.edu).