Imaging Facility

Equipment

Zeiss LSM880 with Airyscan

The Zeiss LSM 880 has two multi-alkali photomultiplier tubes, a GaAsP detector and a transmitted light detector. The addition of the Airyscan provides another GaAsP that may be used when using the Airyscan capability or used as a fourth fluorescence detector in the confocal mode.  The system has six laser lines (405 nm, 458 nm, 488 nm, 514 nm, 561 nm and 633 nm).

For live-cell imaging, the system has motorized z-drive, an environmental chamber and stage incubator for controlling temperature, humidity and CO2.

 

Equipment

Nikon A1R SI Confocal

The Nikon A1RSi confocal microscope is set up on a Nikon Eclipse Ti inverted microscope stand.  The system has four laser lines (405 nm, 488 nm, 561 nm and 640 nm), four photomultiplier tubes for fluorescence, a photomultiplier tube for transmitted light imaging and a spectral detector.

In addition to the galvano scanner, the system has a resonant scanner and heated stage for live-cell imaging.

NIS Elements is used for multi-dimensional imaging and analysis.

A Pico-Quant LSM upgrade kit for FLIM and FCS has been added to the system.

Equipment

Nikon Total Internal Reflected Fluorescence (TIRF) System

The TIRF system is set up on a Nikon Ecliipse Ti microscope.  It has two Andor 897 EMCCD cameras, four laser lines (405 nm, 488 nm, 561 nm and 640 nm) and a FRAP module. The system is run with NIS Elements.

Equipment

Andor Spinning Disk Confocal

The Andor W1 Spinning Disk Confocal is set up on a Nikon Eclipse Ti inverted microscope stand with Perfect Focus.  The system has six laser lines:  405 nm, 445 nm, 488 nm, 560 nm, 637 nm and 685 nm.  Two Andor Zyla cameras allow for two channel simultaneous image capture.  The microscope has an ASI MS-2000 stage that provides fast, repeatable multi-positional imaging.

The spinning disk has a Tokai Hit stage incubator for live-cell imaging.

The system is equipped with an Andor Mosaic photoactivation and photobleaching system as well as a MicroPoint for laser ablation.

 

Equipment

Olympus FVMPE-RV Multiphoton System

This system is set up on a BX63 upright microscope frame with a dual-line Insight 1040/680-1300nm laser and 458 nm diode laser.  It has a resonant scanner, encoded Prior z deck and objective piezo.  There are two multi-alkali photomultipliers and two, GaAsP detectors in the epi-light path as well as a forward detector.

A PicoQuant FCS, FCCS and FLIM upgrade has been added to the system.

The system is run with FluoView software.

Equipment

Nikon Inverted Microscope with Yokogawa W1 spinning disk and wide-field fluorescence and DIC

The Nikon Eclipse Ti-E microscope stand has a Yokogawa, two-camera, CSU-W1 spinning disk system with a Nikon LU-N4 laser launch that includes 405 nm, 488 nm, 561 nm and 640 nm lasers.  The system has Photometrics Prime BSI sCMOS cameras.
 
The microscope stand has Perfect Focus and is equipped with an ASI MS-2000 motorized stage with a piezo Z-drive as well as a Tokai Hit stage top incubation system.

Additionally, the microscope has an Andor Zyla camera for wide-field fluorescence and transmitted light imaging.

Equipment

Fluorescence Stereo Microscope

The Nikon SMZ1500 is equipped for fluorescence (GFP and mCherry).  The microscope has a Spot Insight color camera with Spot software.

Image analysis

Arivis Vision 4D

Arivis Vision 4D software works with 2D, 3D and 4D image data sets from most image formats and can easily manage large data sets.  It is may be used for rendering, stitching, alignment and data analysis including cell segmentation, tracking, annotations, quantitative measurements and statistics.

Image analysis

Imaris Workstation

The facility has an Imaris workstation with the following Imaris components:  Measurement Pro, ImarisTrack, ImarisColoc, ImarisXT, ImarisCell, ImarisVantage and Filament Tracker.  The workstation also has MatLab and has ImageJ installed.

Image analysis

NIS Elements Workstation with Deconvolution

The facility has an NIS Elements analysis workstation.  In addition to the standard NIS Elements features, this workstation is set up for deconvolution.

Image analysis

Zen Blue, Zen Black, NIS Elements and ImageJ Workstation

The facility has an analysis workstation with Zen Blue, Zen Black, NIS Elements and ImageJ.

Training

The resources of the Life Sciences Center Imaging Facility are available to all members of the Dartmouth community and to researchers outside of Dartmouth.

All users of the equipment must be trained before using the equipment.

Please email Ann Lavanway (ann.m.lavanwayATDartmouth.edu).

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